plasmids coding for shrna targeting human foxo1 and scrambled shrna as negative control  (Ribobio co)

Journal: Bone Research

Article Title: Simultaneous augmentation of muscle and bone by locomomimetism through calcium-PGC-1α signaling

doi: 10.1038/s41413-022-00225-w

Figure Lengend Snippet: LAMZ induces Mef2c to enhance myogenesis and osteoblastogenesis. a mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with calcium signal inhibitors together with LAMZ. b mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c . c Representative immunocytofluorescence images of the C2C12 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. Myosin heavy chain (red); nuclei (blue). Scale bar, 100 μm. d mRNA expression of Myog and Ppargc1a . e Representative ALP staining images of the MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. f Representative images of Alizarin Red S staining of MC3T3-E1 cells. g mRNA expression of osteoblastic genes and Ppargc1a . h mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with the PGC-1α inhibitor SR18292. Experiments were repeated 3 times with replicates of two wells. For the comparison of two groups, statistical analyses were carried out using Welch’s t test. For multiple comparisons, two–way ANOVA and Tukey’s multiple-comparison test were applied. The error bars show the mean ± s.e.m. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; N.S. not significant

Article Snippet: The plasmids for producing lentivirus that code shRNAs targeting Mef2c and gfp (control) were purchased from Sigma-Aldrich.

Techniques: Expressing, Infection, Staining

Journal: Nature

Article Title: Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.

doi: 10.1038/nature24023

Figure Lengend Snippet: Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting siRNA; siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;

Article Snippet: The non-coding control shRNA in pLKO.1 was obtained from D. Sabatini via Addgene (Plasmid 186432).

Techniques: Control, Comparison, Immunoprecipitation